Compounds can be identified by measuring Rf values. Column chromatography is a method of separating components within a mixture using a column commonly used in organic chemistry. This separation method is similar to thin layer chromatography, however column chromatography can separate the mixture to a larger volume. In column chromatography, the stationary phase is packed into a glass column. Silica gel was used as the stationary phase in this practice. The more polar compound will remain in the stationary phase for a longer time than a less polar compound. A less polar compound will elute from the column first. The mixture is gently pipetted onto the stationary phase and a mobile phase, known as the eluent, is passed through the column using a pumping system. As the eluent, which in this case is hexane, flows through the column, the mixture separates and the different compounds can be collected when the compound reaches the end of the column (Wilson & Walker, 2010). Factors that affect separation and elution rate include the polarity of the solvent. As the polarity of the mobile phase increases, the substrate travels at a greater speed. Another factor influencing separation includes the interaction of the substrate with the stationary phase where the stronger the interaction, the slower the movement of the substance (Totah, 2011). The main advantage of column chromatography